Article
Experience with 500 prenatal diagnoses of sickle cell diseases: the effect of gestational age on affected pregnancy outcome.
Prenatal diagnosis - 1 Sept 1994
Wang X, Seaman C, Paik M, Chen T, Bank A, Piomelli S
Abstract excerpt
Prenatal diagnosis of sickle cell diseases is obtained rapidly and precisely by polymerase chain reaction (PCR) with Ddel restriction analysis and dot-blotting with allele-specific oligonucleotides (ASO). Prenatal diagnosis of HgbSS and HgbSC was performed in 500 pregnancies, 196 by Southern blot and 304 by PCR. PCR drastically shortened the interval from sampling to reporting, allowing acceptance even of samples...
Topics
- Amniocentesis
- Anemia, Sickle Cell
- Base Sequence
- Blotting, Southern
- Chorionic Villi Sampling
- DNA
- DNA Primers
- Female
- Fetal Diseases
- Gestational Age
- Hemoglobin SC Disease