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Cloning and expression of human neutrophil lipocalin cDNA derived from bone marrow and ovarian cancer cells

9 Jan 1995

Abstract excerpt

Human neutrophil lipocalin (HNL) cDNA was amplified by PCR technology in combination with deoxyinosine containing oligonucleotides for cloning, sequencing and production of the recombinant protein in E. coli. The primers were targeted to the corresponding DNA backtranslate of the mature protein resulting in a PCR amplified 534 bp cDNA from different reverse transcripts of ovarian cancer cell line and bone marrow...

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PMID: 7835423
DOI: 10.1016/0014-5793(94)01303-i

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