Article
Cloning and expression of a cloxacillin-hydrolyzing enzyme and a cephalosporinase from Aeromonas sobria AER 14M in Escherichia coli: requirement for an E. coli chromosomal mutation for efficient expression of the class D enzyme.
Antimicrobial agents and chemotherapy - 1 Sept 1994
Rasmussen B A, Keeney D, Yang Y, Bush K
Abstract excerpt
Two beta-lactamase genes, asbA1 and asbB1, encoding AsbA1 and AsbB1, respectively, have been cloned from Aeromonas sobria AER 14M into Escherichia coli. AsbA1 was expressed at low but detectable levels in all E. coli laboratory cloning strains tested. AsbB1 was expressed well in the E. coli cloning strain DH5 alpha. However, no enzyme activity could be detected from the same clone when placed in E. coli MC1061....
Topics
- Aeromonas
- Amino Acid Sequence
- Base Sequence
- Cephalosporinase
- Chromosomes, Bacterial
- Cloning, Molecular
- Cloxacillin
- Enzyme Stability
- Escherichia coli
- Gene Expression