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Absence of <i>Helicobacter pylori</i> in subgingival samples determined by polymerase chain reaction

1 Oct 1994

Abstract excerpt

The polymerase chain reaction was used for the detection of Helicobacter pylori from subgingival plaque in 336 periodontitis patients. A pair of primers derived from the H. pylori urease gene A served to amplify a targeted 411-bp fragment of genomic DNA. This technique permitted the detection of as few as 60 H. pylori cells. Paper point samples from 3 deep periodontal pockets per patient were immersed in 1 ml of...

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PMID: 7808777
DOI: 10.1111/j.1399-302x.1994.tb00079.x

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