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Quantitation of Helicobacter pylori in dental plaque samples by competitive polymerase chain reaction

1 Mar 2000

Abstract excerpt

AIM: To establish a competitive PCR (cPCR) assay for quantitation of H pylori organisms in dental plaque samples. METHODS: The cPCR co-amplified target H pylori DNA and a known amount of internal standard template in the same tube with the same primers directed to 0.86 kb DNA of H pylori. The internal standard was a synthesised DNA bearing the same primer recognition sites at two ends and a non-homologous core...

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PMID: 10823142
DOI: 10.1136/jcp.53.3.218

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