Article
Detection of fetal HLA-DQa sequences in maternal blood: a gender-independent technique of fetal cell identification.
Prenatal diagnosis - 1 Mar 1995
Geifman-Holtzman O, Holtzman E J, Vadnais T J, Phillips V E, Capeless E L, Bianchi D W
Abstract excerpt
The objective of this study was to detect fetal HLA-DQa gene sequences in maternal blood. HLA-DQa genotypes of 70 pregnant women and their partners were determined for type A1. We specifically sought couples where the father, but not the mother, had genotype A1. In 12 women, maternal blood samples were flow-sorted. Candidate fetal cells were isolated and amplified by using PCR primers specific for a paternal...
Topics
- Base Sequence
- Blood Cells
- Cell Separation
- DNA
- Female
- Fetal Blood
- Flow Cytometry
- Genotype
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- Humans
- Male