Development of PCR assays to detect genetic variation amongst equine herpesvirus-1 isolates as an aid to epidemiological investigation.

doi:10.1016/0166-0934(94)00162-a

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Article

Development of PCR assays to detect genetic variation amongst equine herpesvirus-1 isolates as an aid to epidemiological investigation.

Journal of virological methods - 1 Mar 1995

McCann S H, Mumford J A, Binns M M

Abstract excerpt

A search for variable restriction sites has been carried out for equine herpesvirus-1 (EHV-1) in an attempt to develop markers which can be used to group epidemiologically related viruses into groups, and to learn more about the dynamics of EHV-1 disease. Crude viral DNA extracts of EHV-1, prepared by Hirt extraction, were digested with AluI, HaeIII, or RsaI, and Southern blotted following electrophoresis. DNA...

Topics

Animals
Base Sequence
Cells, Cultured
DNA Primers
DNA Probes
DNA, Viral
Embryo, Mammalian
England
Genetic Variation
Genome, Viral
Herpesviridae Infections

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Herpesvirus 1, Equid

Horse Diseases

Horses

Lung

Molecular Sequence Data

Polymerase Chain Reaction

Restriction Mapping

Source

Open in PubMed

Identifiers

PMID: 7769032
DOI: 10.1016/0166-0934(94)00162-a

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7769032-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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