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Article

Development of a Differential Multiplex PCR Assay for Equine Herpesvirus 1 and 4 as a Diagnostic Tool

1 Jun 2000

Abstract excerpt

In this study, a multiplex polymerase chain reaction (PCR) procedure was developed for differentiation of strains and field isolates of equine herpesvirus type 1 (EHV-1) and type 4 (EHV-4). Specific oli-gonucleotide primers were combined to amplify the thymidine kinase (TK) gene region of EHV-1 and EHV-4, which would yield fragments of different lengths for each virus in the same amplification reaction. The...

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PMID: 10900826
DOI: 10.1046/j.1439-0450.2000.00361.x

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