Article
Phenotypic effects in Saccharomyces cerevisiae after regulated expression of beta-(1,3)-glucanase from Nicotiana plumbaginifolia.
Journal of biotechnology - 1 Feb 1993
Demolder J, De Backer M, Fiers W, Contreras R
Abstract excerpt
A recombinant yeast strain was constructed of which the cell wall porosity could be reversibly and conditionally modulated. This strain expresses the Nicotiana plumbaginifolia beta-(1,3)-glucanase under control of the Saccharomyces cerevisiae GAL1 promoter and the mating factor alpha 1 prepro-sequence. The following phenotypic effects were observed after expression of this enzyme: (a) expressed...
Topics
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Glucan 1,3-beta-Glucosidase
- Glycoside Hydrolases
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- Phenotype
- Plants, Toxic
- Plasmids
- Recombinant Proteins
