Article
Efficient reidentification of chromosome slides from human metaphase spreads via microsatellite polymerase chain reaction.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology - 1 Mar 1995
Epplen C, Epplen J T
Abstract excerpt
In order to exclude inadvertent mixing of slides carrying metaphase spreads, chromosomal DNA was re-extracted several months after routine slide preparation (hypotonic treatment, spreading, fixation, staining, embedding and microscopic inspection) in different laboratories. The DNA yield was largely dependent on the number of cells originally fixed and the batch of the embedding material. Average fragment sizes...
Topics
- Alleles
- Blood Cells
- Chromosomes, Human
- DNA, Satellite
- Gene Frequency
- Histocytological Preparation Techniques
- Humans
- Polymerase Chain Reaction
- Quality Control
- Single-Blind Method