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Article

Genetic analysis of the fluorescein isothiocyanate binding site of the yeast plasma membrane H(+)-ATPase.

The Journal of biological chemistry - 14 Apr 1995

Maldonado A M, Portillo F

Abstract excerpt

The highly conserved motif of Saccharomyces cerevisiae H(+)-ATPase 474KGAP has been proposed to participate in the formation of the phosphorylated intermediate during the catalytic cycle (Portillo, F., and Serrano, R. (1988) EMBO J. 7, 1793-1798). In addition, Lys-474 is the FITC binding site of the yeast enzyme (Portillo, F. and Serrano, R. (1989) Eur. J. Biochem. 186, 501-507). We have performed an intragenic...

Topics

Binding Sites
Cell Membrane
Fluorescein-5-isothiocyanate
Hydrogen-Ion Concentration
Mutagenesis, Site-Directed
Phenotype
Proton-Translocating ATPases
Saccharomyces cerevisiae

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 7721767
DOI: 10.1074/jbc.270.15.8655

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7721767-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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