Article
The identification of bacterial gene expression differences using mRNA-based isothermal subtractive hybridization.
Canadian journal of microbiology - 1 Feb 1995
Utt E A, Brousal J P, Kikuta-Oshima L C, Quinn F D
Abstract excerpt
We describe a method for isolating and determining differences in gene expression between related bacterial strains. The method is based upon differences in mRNA expression. To demonstrate this procedure, cDNA generated from total RNA of Listeria monocytogenes serotype 1/2a was hybridized to total RNA from a Tn916 mutant of serogroup 1/2a (M3) that was deficient in the production of listeriolysin O, the product...
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