The identification of bacterial gene expression differences using mRNA-based isothermal subtractive hybridization.

doi:10.1139/m95-020

Back to search

Article

The identification of bacterial gene expression differences using mRNA-based isothermal subtractive hybridization.

Canadian journal of microbiology - 1 Feb 1995

Utt E A, Brousal J P, Kikuta-Oshima L C, Quinn F D

Abstract excerpt

We describe a method for isolating and determining differences in gene expression between related bacterial strains. The method is based upon differences in mRNA expression. To demonstrate this procedure, cDNA generated from total RNA of Listeria monocytogenes serotype 1/2a was hybridized to tota...

Topics

Base Sequence
DNA, Complementary
DNA, Single-Stranded
Gene Expression Regulation, Bacterial
Listeria monocytogenes
Molecular Sequence Data
Mutation
Nucleic Acid Hybridization
RNA, Messenger

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.