Article
Application of capillary high-performance liquid chromatography to biotechnology, with reference to the analysis of recombinant DNA-derived human growth hormone.
Journal of chromatography. B, Biomedical applications - 9 Dec 1994
Battersby J E, Guzzetta A W, Hancock W S
Abstract excerpt
Using capillary HPLC, femtomole amounts of recombinant DNA-derived human growth hormone (rhGH) have been successfully detected from solutions at nanomolar concentrations. The separation used capillaries of 15 cm x 320 microns I.D. and detection was with a UV absorbance detector containing a capil...
Topics
- Amino Acid Sequence
- Biotechnology
- Chromatography, High Pressure Liquid
- Chromatography, Ion Exchange
- DNA, Recombinant
- Growth Hormone
- Humans
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutation
- Peptide Mapping
- Recombinant Proteins
- Spectrophotometry, Ultraviolet
- Trypsin
