Article
Accurate size comparison of short tandem repeat alleles amplified by PCR.
BioTechniques - 1 Jan 1995
Smith R N
Abstract excerpt
A strategy is presented for classifying complex short tandem repeat (STR) alleles by size. Such alleles can differ in length by only 1 bp. The HUMACTBP2 locus was used as a model. Dye-labeled, PCR-amplified alleles were analyzed on an automated DNA sequencer with laser-induced fluorescence detection and fragment-sizing software. Between-gel allele sizes calculated against an in-lane allelic ladder or viral DNA...
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