Optimization of a method for simultaneous analysis of cell surface phenotype and cell cycle in human bone marrow and peripheral blood leukocytes by flow cytometry: the value of both internal and external standards.

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Article

Optimization of a method for simultaneous analysis of cell surface phenotype and cell cycle in human bone marrow and peripheral blood leukocytes by flow cytometry: the value of both internal and external standards.

Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology - 1 Jul 1993

Jensen I M, Kristensen J S, Thomsen M, Ellegaard J, Hokland P

Abstract excerpt

Three different methods for the simultaneous analysis of surface phenotype and DNA quantification were compared. One method, involving the fixation of cells in 70% ethanol, was convincingly superior, both with regard to the CV of the G0G1 peak and the intensity of the DNA labelling. Furthermore, the correlation between the surface antigen densities before and after fixation were high. Experiments evaluating the...

Topics

Animals
Bone Marrow Cells
Cell Cycle
Cell Membrane
Chickens
DNA
Female
Flow Cytometry
Humans
Leukocytes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Male

Phenotype

Ploidies

Reference Standards

Staining and Labeling

Tissue Fixation

Source

Open in PubMed

Identifiers

PMID: 7689857

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7689857-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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