Article
Nitrogen regulation in an Escherichia coli strain with a temperature sensitive glutamyl-tRNA synthetase.
Molecular & general genetics : MGG - 1 Jun 1993
Osorio A V, Camarena L, Salazar G, Noll-Louzada M, Bastarrachea F
Abstract excerpt
Escherichia coli cells carrying the gltX351 allele are unable to grow at 42 degrees C (Ts phenotype) due to an altered glutamyl-tRNA synthetase. We found that gltX351 cells display a new phenotype termed Gsd-, i.e. an inability to raise glutamine synthetase activity above low constitutive levels in minimal medium with 6.8 mM glutamine as sole nitrogen source. When 0.5 mM NH4+ or 12 mM glutamate replaced...
Topics
- Enzyme Repression
- Escherichia coli
- Gene Conversion
- Genes, Bacterial
- Genes, Dominant
- Genes, Regulator
- Glutamate-tRNA Ligase
- Ketoglutaric Acids
- Mutagenesis, Insertional
- Mutation
- Nitrogen