Article
[Mutation detection by PCR-TGGE].
Rinsho byori. The Japanese journal of clinical pathology - 1 Jul 1995
Shirakawa T, Nishiyama K, Matsuo M
Abstract excerpt
The variants in enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) gene were detected by temperature gradient gel electrophoresis (TGGE). 15 clinical strains isolated from patients and a wild type strain (B2C) were analyzed after the conventional PCR. Although all PCR products (707bp) corresponded to A-subunit of LT, three strains were the different electrophoretic patterns after silver staining as...
Topics
- Base Sequence
- Electrophoresis
- Enterotoxins
- Escherichia coli
- Genetic Variation
- Hot Temperature
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction