Detection of two variant Vero toxin genes in Escherichia coli by capillary electrophoresis.

doi:10.1002/bmc.130

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Article

Detection of two variant Vero toxin genes in Escherichia coli by capillary electrophoresis.

Biomedical chromatography : BMC - 1 Feb 2002

Arakawa Hidetoshi, Watanabe Kazuyuki, Kashiwazaki Hiroyuki, Maeda Masako

Abstract excerpt

Three methods [capillary electrophoresis (CE)-allele-specific PCR, CE-single-strand conformation polymorphism (SSCP) and CE-cleavase fragment length polymorphism (CFLP)] were developed in order to effect rapid and specific analysis of the vero toxin (VT)1 and VT2 genes of O157. The allele-specific polymerase chain reaction (PCR) method, which utilized specific duplex PCR with specific primers for VT1 and VT2,...

Topics

Alleles
Base Sequence
DNA Primers
Electrophoresis, Capillary
Escherichia coli
Lasers
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Protein Isoforms
Shiga Toxins
Spectrometry, Fluorescence

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Identifiers

PMID: 11816010
DOI: 10.1002/bmc.130

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