Article
Detection of two variant Vero toxin genes in Escherichia coli by capillary electrophoresis.
Biomedical chromatography : BMC - 1 Feb 2002
Arakawa Hidetoshi, Watanabe Kazuyuki, Kashiwazaki Hiroyuki, Maeda Masako
Abstract excerpt
Three methods [capillary electrophoresis (CE)-allele-specific PCR, CE-single-strand conformation polymorphism (SSCP) and CE-cleavase fragment length polymorphism (CFLP)] were developed in order to effect rapid and specific analysis of the vero toxin (VT)1 and VT2 genes of O157. The allele-specific polymerase chain reaction (PCR) method, which utilized specific duplex PCR with specific primers for VT1 and VT2,...
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