Article
Genetic diversity in the non-structural gene of parvovirus B19 detected by single-stranded conformational polymorphism assay (SSCP) and partial nucleotide sequencing.
Journal of virological methods - 1 Jun 1995
Kerr J R, Curran M D, Moore J E, Erdman D D, Coyle P V, Nunoue T, Middleton D, Ferguson W P
Abstract excerpt
A homologous region in the parvovirus B19 non-structural gene (B19 nt 1399-1682) was examined in 50 samples from patients with a wide variety of B19-related disease from various countries by PCR amplification, single-stranded conformational polymorphism (SSCP) assay and nucleotide sequencing. Five SSCP types were confirmed by nucleotide sequence analysis. Of a total of 6 mutations, all were silent. Types 3 and 4...
Topics
- Adolescent
- Adult
- Aged
- Child
- Child, Preschool
- DNA, Viral
- Erythema Infectiosum
- Female
- Genetic Variation
- Humans
- Infant
