Article
Protein exporter function and in vitro ATPase activity are correlated in ABC-domain mutants of HlyB.
Molecular microbiology - 1 Apr 1995
Koronakis E, Hughes C, Milisav I, Koronakis V
Abstract excerpt
The Escherichia coli toxin exporter HlyB comprises an integral membrane domain fused to a cytoplasmic domain of the ATP-binding cassette (ABC) super-family, and it directs translocation of the 110kDa haemolysin protein out of the bacterial cell without using an N-terminal secretion signal peptide...
Topics
- ATP-Binding Cassette Transporters
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Amino Acid Sequence
- Endopeptidase K
- Escherichia coli
- Hemolysin Proteins
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Recombinant Fusion Proteins
- Restriction Mapping
- Serine Endopeptidases
- Structure-Activity Relationship