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Article

Protein exporter function and in vitro ATPase activity are correlated in ABC-domain mutants of HlyB.

Molecular microbiology - 1 Apr 1995

Koronakis E, Hughes C, Milisav I, Koronakis V

Abstract excerpt

The Escherichia coli toxin exporter HlyB comprises an integral membrane domain fused to a cytoplasmic domain of the ATP-binding cassette (ABC) super-family, and it directs translocation of the 110kDa haemolysin protein out of the bacterial cell without using an N-terminal secretion signal peptide. We have exploited the ability to purify the soluble HlyB ABC domain as a fusion with glutathione S-transferase to...

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Identifiers

PMID: 7651140
DOI: 10.1111/j.1365-2958.1995.tb02394.x

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