Article
Assay for movement of Lepidopteran transposon IFP2 in insect cells using a baculovirus genome as a target DNA.
Virology - 20 Aug 1995
Fraser M J, Cary L, Boonvisudhi K, Wang H G
Abstract excerpt
Mutagenesis of baculoviruses by host mobile elements occurs spontaneously and frequently during propagation of the viruses in Lepidopteran cell cultures. Most of the transposons identified as insertions in baculovirus genomes are relatively small Class II elements that exhibit a remarkable specificity for TTAA target sites. We have developed a transposition assay to analyze the movement of these TTAA-specific...
Topics
- Animals
- Base Sequence
- Cells, Cultured
- DNA Transposable Elements
- DNA, Viral
- Genome, Viral
- Lepidoptera
- Molecular Sequence Data
- Mutagenesis
- Nucleopolyhedroviruses
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