Article
Solution characterisation by NMR spectroscopy of two horseradish peroxidase isoenzyme C mutants with alanine replacing either Phe142 or Phe143.
European journal of biochemistry - 15 Oct 1995
Veitch N C, Williams R J, Bone N M, Burke J F, Smith A T
Abstract excerpt
Site-directed mutagenesis of the horseradish peroxidase isoenzyme C (HRP C) gene has been undertaken in order to provide two recombinant enzymes where alanine replaces either Phe142 or Phe143 ([F142A]HRP C and [F143A]HRP C, respectively). These heme enzymes have been characterised in solution usi...
Topics
- Alanine
- Base Sequence
- Binding Sites
- Horseradish Peroxidase
- Isoenzymes
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Phenylalanine
- Solutions
- Structure-Activity Relationship