Article
Structure and function of mutant Arg44Lys of 4-hydroxybenzoate hydroxylase implications for NADPH binding.
European journal of biochemistry - 1 Jul 1995
Eppink M H, Schreuder H A, Van Berkel W J
Abstract excerpt
Arg44, located at the si-face side of the flavin ring in 4-hydroxybenzoate hydroxylase, was changed to lysine by site-specific mutagenesis. Crystals of [R44K]4-hydroxybenzoate hydroxylase complexed with 4-hydroxybenzoate diffract to 0.22-nm resolution. The structure of [R44K]4-hydroxybenzoate hyd...
Topics
- 4-Hydroxybenzoate-3-Monooxygenase
- Arginine
- Base Sequence
- Binding Sites
- Catalysis
- Lysine
- Molecular Sequence Data
- Mutation
- NADP
- Oligodeoxyribonucleotides
- Spectrum Analysis
- Structure-Activity Relationship