Article
Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate,2,4-dihydroxybenzoate, and 2-hydroxy-4-aminobenzoate and of the Tyr222Ala mutant complexed with 2-hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring.
Biochemistry - 23 Aug 1994
Schreuder H A, Mattevi A, Obmolova G, Kalk K H, Hol W G, van der Bolt F J, van Berkel W J
Abstract excerpt
The crystal structures of wild-type p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens, complexed with the substrate analogues 4-aminobenzoate, 2,4-dihydroxybenzoate, and 2-hydroxy-4-aminobenzoate have been determined at 2.3-, 2.5-, and 2.8-A resolution, respectively. In addition, the cry...
Topics
- 4-Aminobenzoic Acid
- 4-Hydroxybenzoate-3-Monooxygenase
- Alanine
- Aminosalicylic Acid
- Base Sequence
- Benzoates
- Binding Sites
- Crystallization
- Crystallography, X-Ray
- Flavins
- Hydrogen Bonding
- Hydroxybenzoates
- Ion Channels