Article
Selection of Mycoplasma hominis PG21 deletion mutants by cultivation in the presence of monoclonal antibody 552.
Infection and immunity - 1 Sept 1995
Jensen L T, Ladefoged S, Birkelund S, Christiansen G
Abstract excerpt
Three mutants of Mycoplasma hominis PG21 were isolated and shown to contain alterations in the size of a repeat-containing gene encoding a surface-localized 135-kDa antigen designated Lmp1. The mutants were isolated by cultivating M. hominis for a 3-month period in the presence of Lmp1-specific monoclonal antibody (MAb) 552. The epitope for MAb 552 was localized at the repeated part of the protein. The gene...
Topics
- Agglutination Tests
- Antibodies, Monoclonal
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- Electrophoresis, Gel, Pulsed-Field
- Molecular Sequence Data
- Mutation
- Mycoplasma
- RNA, Bacterial
- Repetitive Sequences, Nucleic Acid