Article
Processing of long-stored archival cervical smears for human papillomavirus detection by the polymerase chain reaction.
British journal of cancer - 1 Aug 1995
de Roda Husman A M, Snijders P J, Stel H V, van den Brule A J, Meijer C J, Walboomers J M
Abstract excerpt
The efficiency of a freeze-thaw method, a proteinase K/Tween 20 lysis method and a guanidinium isothiocyanate/silica beads method for DNA extraction from fixed and Papanicolaou-stained cells from the cervical cancer cell line Siha was measured by beta-globin polymerase chain reaction (PCR). The GTC/silica beads method, which appeared superior, revealed a human papillomavirus (HPV) general primer-mediated PCR...
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