Article
Processing of long-stored archival cervical smears for human papillomavirus detection by the polymerase chain reaction.
British journal of cancer - 1 Aug 1995
de Roda Husman A M, Snijders P J, Stel H V, van den Brule A J, Meijer C J, Walboomers J M
Abstract excerpt
The efficiency of a freeze-thaw method, a proteinase K/Tween 20 lysis method and a guanidinium isothiocyanate/silica beads method for DNA extraction from fixed and Papanicolaou-stained cells from the cervical cancer cell line Siha was measured by beta-globin polymerase chain reaction (PCR). The G...
Topics
- Base Sequence
- Beta-Globulins
- Biopsy
- DNA
- DNA, Viral
- Endopeptidase K
- Evaluation Studies as Topic
- Feasibility Studies
- Female
- Freezing
- Genotype
- Guanidines
- Humans
- Isothiocyanates
- Molecular Sequence Data
- Papanicolaou Test
- Papillomaviridae
- Polymerase Chain Reaction