Article
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
Microbiological reviews - 1 Mar 1995
Amann R I, Ludwig W, Schleifer K H
Abstract excerpt
The frequent discrepancy between direct microscopic counts and numbers of culturable bacteria from environmental samples is just one of several indications that we currently know only a minor part of the diversity of microorganisms in nature. A combination of direct retrieval of rRNA sequences and whole-cell oligonucleotide probing can be used to detect specific rRNA sequences of uncultured bacteria in natural...
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