Article
Genotyping of hepatitis C virus isolates by a modified polymerase chain reaction assay using type specific primers: epidemiological applications.
Journal of medical virology - 1 Nov 1994
Widell A, Shev S, Månsson S, Zhang Y Y, Foberg U, Norkrans G, Frydén A, Weiland O, Kurkus J, Nordenfelt E
Abstract excerpt
A polymerase chain reaction (PCR)-based assay using primers against the hepatitis C core gene has been described [Okamoto et al. (1992a): Journal of General Virology 73:673-679]. Within the two major HCV genotypes 1 and 2, the Okamoto system identifies two subtypes each (1a, 1b and 2a, 2b, respectively). Typing is achieved by a primary PCR with consensus primers followed by a nested PCR with type specific...
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