Article
Cytoplasmic free-Ca2+ level rises with repellents and falls with attractants in Escherichia coli chemotaxis.
Proceedings of the National Academy of Sciences of the United States of America - 7 Nov 1995
Tisa L S, Adler J
Abstract excerpt
Cytoplasmic free-Ca2+ levels in Escherichia coli were measured by use of the fluorescent Ca(2+)-indicator dye fura-2. Chemotactically wild-type E. coli regulated cytoplasmic free Ca2+ at approximately 100 nM when no stimuli were encountered, but changes in bacterial behavior correlated with changes in cytoplasmic free-Ca2+ concentration. For chemotactically wild-type E. coli, addition of a repellent resulted in...
Topics
- Amino Acids
- Calcium
- Chemotaxis
- Cytoplasm
- Electroporation
- Escherichia coli
- Fura-2
- Mutation
- Receptors, Cell Surface
- Spectrometry, Fluorescence