Article
Selection of motile nonchemotactic mutants of Escherichia coli by field-flow fractionation.
Proceedings of the National Academy of Sciences of the United States of America - 15 Sept 1991
Berg H C, Turner L
Abstract excerpt
We have developed a chromatographic method for isolating bacterial cells that are motile but nonchemotactic. Separation of strains of different phenotype occurs along a thin horizontal channel between two stirred chambers, the lower one containing a chemical attractant. The channel is bounded above and below by rigid filters, permeable to the attractant but not to the bacteria. The lower part of the channel is...
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