Selection of motile nonchemotactic mutants of Escherichia coli by field-flow fractionation.

doi:10.1073/pnas.88.18.8145

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Article

Selection of motile nonchemotactic mutants of Escherichia coli by field-flow fractionation.

Proceedings of the National Academy of Sciences of the United States of America - 15 Sept 1991

Berg H C, Turner L

Abstract excerpt

We have developed a chromatographic method for isolating bacterial cells that are motile but nonchemotactic. Separation of strains of different phenotype occurs along a thin horizontal channel between two stirred chambers, the lower one containing a chemical attractant. The channel is bounded abo...

Topics

Cell Separation
Chemotaxis
Escherichia coli
Movement
Mutation

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