Article
Fractionation of non allelic histone variants by hydrophobic interaction chromatography.
Preparative biochemistry - 1 Jan 2000
Ivanova V S
Abstract excerpt
A method for the separation of non allelic histone variants is described. Sepharose-CL-4B was modified with dodecylamine by the carbonyldiimidazole-method. The matrix prepared contained 6 mumol/ml hydrophobic groups, C12-hydrocarbon chains. Proteins were loaded at high ionic strength and eluted f...
Topics
- Animals
- Carcinoma, Ehrlich Tumor
- Cattle
- Chemical Fractionation
- Chromatin
- Chromatography
- Genetic Variation
- Histones
- Liver
- Osmolar Concentration
- Rats
- Thymus Gland
- Urea
