Article
Fractionation of non allelic histone variants by hydrophobic interaction chromatography.
Preparative biochemistry - 1 Jan 2000
Ivanova V S
Abstract excerpt
A method for the separation of non allelic histone variants is described. Sepharose-CL-4B was modified with dodecylamine by the carbonyldiimidazole-method. The matrix prepared contained 6 mumol/ml hydrophobic groups, C12-hydrocarbon chains. Proteins were loaded at high ionic strength and eluted from the column with an urea gradient. Histones H1, H2a and H2b from different mammalian tissues were loaded on the...
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