Separation of rat tissue histone H1 subtypes by reverse-phase h.p.l.c. Identification and assignment to a standard H1 nomenclature.

doi:10.1042/bj2690359

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Article

Separation of rat tissue histone H1 subtypes by reverse-phase h.p.l.c. Identification and assignment to a standard H1 nomenclature.

The Biochemical journal - 15 Jul 1990

Lindner H, Helliger W, Puschendorf B

Abstract excerpt

H1 histones from rat liver and rat testis were separated by reverse-phase h.p.l.c. Within 40 min six subfractions (H1(0), H1b, H1a, H1d, H1e + H1c and H1c) and seven subfractions (H1(0), H1b, H1a, H1d, H1e + H1c, H1c and H1t) respectively were isolated by using a linear acetonitrile gradient. Each individual H1 subtype was identified either by comparing the H1 variants (contained in both tissues but in different...

Topics

Acetates
Acetic Acid
Amino Acids
Animals
Cell Nucleus
Chemical Fractionation
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Genetic Variation
Histones
Liver

Male

Rats

Sodium Dodecyl Sulfate

Testis

Urea

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