Article
Red cell phenotyping using hexadimethrine bromide (Polybrene) in a microplate system.
Transfusion - 1 Jan 2000
Anderson H J, Patel S
Abstract excerpt
To provide rapidly phenotyped units of blood, we adapted the hexadimethrine bromide (Polybrene) technique to microplate technology. Pilot samples from 282 donor units were phenotyped for antigens in the Rh, Kidd, Kell, Duffy, and Ss systems with a standard tube-testing method and a Polybrene-microplate (P-MP) technique. Diluted antisera and a 1 percent red cell suspension were used to give P-MP reactions that...
Topics
- Blood Group Antigens
- Blood Grouping and Crossmatching
- Coombs Test
- False Negative Reactions
- False Positive Reactions
- Hemagglutination Tests
- Hexadimethrine Bromide
- Humans
- Phenotype
- Polyamines