Article
A novel assay for typing Rh antigens in blood-stains using a lectin specific to the bisecting N-acetyl-D-glucosamine side chain of glycoprotein.
Journal of immunological methods - 1 Aug 1994
Matsubara K, Tanabe K, Akane A, Nakamura H, Takahashi S, Kimura K
Abstract excerpt
A unique sandwich enzyme-linked immunosorbent assay for the determination of Rh antigens in blood stains has been developed using Rh antisera and phaseolus vulgaris E4/peroxidase conjugate (PHAE4/PO). The appropriate antiserum for detecting Rh C, c, D, E or e was coated on the inner surface of mi...
Topics
- Acetylglucosamine
- Antigen-Antibody Complex
- Blood Grouping and Crossmatching
- Blood Preservation
- Blood Stains
- Cross Reactions
- Enzyme-Linked Immunosorbent Assay
- Genotype
- Humans
- Immune Sera
- Phytohemagglutinins
- Rh-Hr Blood-Group System
- Solubility
