Article
Not more than 117 base pairs of 5'-flanking sequence are required for inducible expression of a human IFN-alpha gene.
Nature - 1 Jan 2000
Ragg H, Weissmann C
Abstract excerpt
Interferon genes are usually only expressed after induction. In the accompanying paper we have shown that the accumulation of mRNA after viral induction is due to activation of transcription, rather than to reduction of turnover, and that the regulation of the alpha-interferon (IFN-alpha) gene is mediated by a segment of 5'-flanking region of not more than 700 base pairs (bp). To delineate the sequences required...
Topics
- Animals
- Base Composition
- Base Sequence
- DNA Restriction Enzymes
- Genes
- Humans
- Interferon Type I
- L Cells
- Mice
- Mutation
- Plasmids
- Transcription, Genetic