Article
Correlation of biophysical properties and cell surface antigenic profile of Percoll gradient-separated human natural killer cells.
Natural immunity and cell growth regulation - 1 Jan 2000
Phillips J H, Warner N L, Lanier L L
Abstract excerpt
Human peripheral blood nylon wool nonadherent lymphocytes were fractionated according to cellular density on discontinuous Percoll gradients. The various fractions of cells obtained by this procedure were then analyzed to correlate morphology, cytotoxicity, light scatter properties and antigenic profile. The majority of natural killer (NK) cell activity was manifested by light density lymphocytes, which...
Topics
- Antibodies, Monoclonal
- Antigens, Surface
- Cell Separation
- Flow Cytometry
- Humans
- Killer Cells, Natural
- Phenotype
- Povidone
- Silicon Dioxide