Article
[Direct selection of Aspergillus terricola variants having different toxicity on a culture of fibroblasts].
Mikrobiologiia - 1 Jan 2000
Imshenetskii A A, Solntseva L I, Kasatkina I D, Zheltova E T, Afanas'eva L N
Abstract excerpt
A direct method is proposed to select less toxic mutants of Aspergillus terricola on a culture of fibroblasts. The conidia are first irradiated with UV, and then are used to grow colonies on the glass surface of tubes or flasks containing medium 199. The cells of fibroblasts are added thereupon and the two cultures are being grown together for 24--48 hours. The colonies which inhibit the growth of fibroblasts to...
Topics
- Aspergillus
- Cell Survival
- Fibroblasts
- Genetic Variation
- Methods
- Mutation
- Mycotoxins
- Species Specificity