Multilocus Oxford Nanopore sequencing reveals genetic diversity of Giardia duodenalis and Cryptosporidium spp. in surface and wastewater in Southwestern Colombia.

doi:10.1186/s13071-026-07565-0

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Multilocus Oxford Nanopore sequencing reveals genetic diversity of Giardia duodenalis and Cryptosporidium spp. in surface and wastewater in Southwestern Colombia.

Parasites & vectors - 26 Aug 2026

Gutiérrez Stivenn, Urrea Vanessa, Patiño Luz H, Hidalgo-Troya Arsenio, Galeano Luis Alejandro, Muñoz Marina, Ramírez Juan David

Abstract excerpt

BACKGROUND: Giardia duodenalis and Cryptosporidium spp. are globally significant enteric protozoa responsible for waterborne diarrheal disease with important public health and zoonotic implications. Conventional genotyping approaches based on Sanger sequencing frequently underestimate mixed infections and minority variants, limiting the resolution of environmental molecular surveillance. This study aimed to...

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Topics

Cryptosporidium
Giardia lamblia
Genetic Variation
Colombia
Wastewater
RNA, Ribosomal, 18S

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Nanopore Sequencing

Triose-Phosphate Isomerase

DNA, Protozoan

Glutamate Dehydrogenase

Multilocus Sequence Typing

Giardiasis

Cryptosporidiosis

Phylogeny

Genotype

Humans

Source

Open in PubMed Open full text

Identifiers

PMID: 42754890
DOI: 10.1186/s13071-026-07565-0
PMCID: PMC13587459

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