Article
Enhancing Trypanosomatid Identification and Genotyping with Oxford Nanopore Sequencing: Development and Validation of an 18S rRNA Amplicon-Based Method.
The Journal of molecular diagnostics : JMD - 1 May 2024
Cruz-Saavedra Lissa, Ospina Carlos, Patiño Luz H, Villar Juan C, Sáenz Pérez Luis D, Cantillo-Barraza Omar, Jaimes-Dueñez Jeiczon, Ballesteros Nathalia, Cáceres Tatiana, Vallejo Gustavo, Ramírez Juan D
Abstract excerpt
Trypanosomatids, including Trypanosoma and Leishmania species, present significant medical and veterinary challenges, causing substantial economic losses, health complications, and even fatalities. Diagnosing and genotyping these species and their genotypes is often complex, involving multiple steps. This study aimed to develop an amplicon-based sequencing (ABS) method using Oxford Nanopore long-read sequencing...
Topics
- Humans
- Animals
- Genotype
- RNA, Ribosomal, 18S
- Nanopore Sequencing
- Coinfection
- Chagas Disease
- Trypanosoma cruzi