Article
OmrA sRNA inhibits translation of phosphoenolpyruvate carboxylase to impair TCA-cycle flux.
Nucleic acids research - 10 Aug 2026
Stenum Thomas, Le Huyen Kim Boi, Kjellin Jonas, Koskiniemi Sanna, Wagner E Gerhart H, Holmqvist Erik
Abstract excerpt
Small RNAs (sRNAs) rarely cause strong growth phenotypes upon overexpression, complicating efforts to link regulatory interactions to physiological outcomes. Here, we report that high levels of the Escherichia coli sRNA OmrA, but not its sibling OmrB, severely inhibit growth in glucose minimal medium. Genetic, biochemical, and physiological analyses indicate that OmrA-dependent toxicity results from reduced flux...
Topics
- Citric Acid Cycle
- Phosphoenolpyruvate Carboxylase
- Escherichia coli
- RNA, Small Untranslated
- Escherichia coli Proteins
- Protein Biosynthesis
- Mutation
- Host Factor 1 Protein
- Gene Expression Regulation, Bacterial
- 5' Untranslated Regions