Article
MiaA-mediated tRNA modifications couple tryptophan attenuation and changes in tRNA abundance to complex phenotypes in Pseudomonas aeruginosa.
Nucleic acids research - 17 Jul 2026
Frommeyer Yannick N, Thöming Janne G, Gomez Nicolas O, Grobe Svenja, Preusse Matthias, Arce-Rodríguez Alejandro, Neubauer Kerstin, Kennepohl Benedikt, Kirk Tim, Franke Raimo, Brönstrup Mark, Neumann-Schaal Meina, Müsken Mathias, Depledge Daniel P, Bähre Heike, Pich Andreas, Häussler Susanne
Abstract excerpt
Transfer RNA (tRNA)-modifying enzymes are emerging as key regulators of bacterial physiology. MiaA, a tRNA isopentenyltransferase, is well studied in model organisms, but its role in the opportunistic pathogen Pseudomonas aeruginosa remains unclear. Using LC-MS, nanopore tRNA sequencing, as well as transcriptional, translational, and proteomic profiling, we mapped MiaA-dependent tRNA modifications and revealed...
Topics
- Pseudomonas aeruginosa
- Tryptophan
- RNA, Transfer
- Phenotype
- Bacterial Proteins
- Virulence
- Animals
- Gene Expression Regulation, Bacterial
- Protein Biosynthesis