High-content imaging driven algal phenotypes enable precise multilevel discrimination of heavy metal stress.

Abstract excerpt

Conventional water quality monitoring based on fixed chemical thresholds often fails to capture the integrated biological effects of pollutant mixtures and sublethal stress. To address this limitation, we developed a high-content imaging (HCI)-based pipeline that converts phenotypic changes in algal cells into multidimensional quantitative features for toxicity assessment. A heavy metal stress model was...

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