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Article

Development of a High-Sensitivity Multiplex mRNA-Based KIT D816V Droplet Digital PCR Assay and Correlation with Tumor Load in Systemic Mastocytosis.

International journal of molecular sciences - 16 Jul 2026

Alheraky Abdulrazzaq, Simpelaar Arjan, Hesp Lucy B, Wierenga Albertus T J, Heddema Moniek, Minović Isidor, Klein Saskia K, Oude Elberink Hanneke N G, Kema Ido P, Mulder André B

Abstract excerpt

Detecting a KIT D816V mutation in patients with systemic mastocytosis (SM) needs a high-sensitivity assay. Additionally, high mutant KIT transcriptional activity and multilineage involvement are strong unfavorable prognostic parameters in SM. The current frequently used quantitative PCR-based KIT D816V assays have important analytical limitations, including considerable analytical variation in the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 42511657
DOI: 10.3390/ijms27146314
PMCID: PMC13410330

Genes

KIT
ABL1

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

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