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CRISPR/Cas9-Mediated Knockout of White Gene Produces Eye Color Mutants in the Southern Green Stinkbug, Nezara viridula.

Archives of insect biochemistry and physiology - 1 Jun 2026

Sengodan Karthi, Xian Chen, Shirk Paul, Howell Jeff, Moola Anil Kumar, Palli Subba Reddy

Abstract excerpt

CRISPR/Cas9 gene editing is a transformative tool for genetic studies in non-model organisms like the southern green stinkbug, Nezara viridula. However, current protocols depend on embryonic microinjection of CRISPR/Cas9, which remains technically difficult. An alternative method of delivering Cas9 ribonucleoprotein directly into female ovaries has been tested in only a few insect species, such as mosquitoes and...

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PMID: 42251734
DOI: 10.1002/arch.70175

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