Article
Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing.
Nature communications - 1 Aug 2018
Chaverra-Rodriguez Duverney, Macias Vanessa M, Hughes Grant L, Pujhari Sujit, Suzuki Yasutsugu, Peterson David R, Kim Donghun, McKeand Sage, Rasgon Jason L
Abstract excerpt
Cas9-mediated gene editing is a powerful tool for addressing research questions in arthropods. Current approaches rely upon delivering Cas9 ribonucleoprotein (RNP) complex by embryonic microinjection, which is challenging, is limited to a small number of species, and is inefficient even in optimized taxa. Here we develop a technology termed Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) to deliver...
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