Development and application of a SNP-based strain-specific identification PCR method for Bifidobacterium animalis subsp. lactis BL-99 using PCR coupled with sanger sequencing.

Abstract excerpt

Accurate strain-specific identification of probiotics is critical for ensuring product efficacy and safety. However, many established techniques, including whole-genome sequencing, pose challenges for industrial application due to their cost, speed, and practicality limitations. To resolve this gap, we developed a novel strain-specific PCR coupled with Sanger sequencing method for Bifidobacterium animalis subsp....

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to DNA, Bacterial
• Share to DNA Primers