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Mutations adjacent to the nucleotide-binding cleft of Arabidopsis thaliana ACTIN7 confer resistance to the actin-disrupting compound latrunculin B.

Journal of experimental botany - 16 Dec 2025

Sparks J Alan, Sun Liang, Chin Sabrina, Ramanjulu Nolan, Wen Jiangqi, Gilroy Simon, Blancaflor Elison B, Khan Bibi Rafeiza

Abstract excerpt

A forward genetic screen identified a dominant-negative Arabidopsis thaliana mutant resistant to growth inhibition caused by the actin-disrupting compound latrunculin B (LatB). Map-based cloning and whole-genome sequencing revealed that the mutant referred to here as lbr1 for LatB resistant1 had a point mutation in the AT5G09810 gene, which encodes the vegetative actin (ACT) isoform ACT7. The cytosine to thymine...

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PMID: 40747687
DOI: 10.1093/jxb/eraf346

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