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Development and evaluation of a triplex real-time PCR assay for enhanced plague diagnostics in Madagascar

24 Jul 2025

Abstract excerpt

BACKGROUND: The plague, caused by Yersinia pestis, remains a critical public health issue, particularly in endemic regions like Madagascar. Rapid and accurate detection of this pathogen is essential for effective outbreak management and timely intervention. Following the urban plague outbreak of 2017, a new molecular diagnostic algorithm was developed and introduced into routine use. However, certain cases...

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PMID: 40705833
DOI: 10.1371/journal.pntd.0013278

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