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The updated duplex fluorescence quantitative RT-PCR assay for simultaneous detection of PRRSV-1 and PRRSV-2

19 Jun 2025

Abstract excerpt

Introduction: Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating infectious diseases in the global swine industry. With the continuous mutation and recombination of PRRSV, existing detection methods frequently result in false negatives, further complicating the prevention and control of PRRS. Methods: The duplex real-time quantitative RT-PCR (RT-qPCR) for the...

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PMID: 40612389
DOI: 10.3389/fcimb.2025.1616898

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