Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Development of a novel duplex crystal digital PCR for the detection of PRRSV-1 and PRRSV-2.

Frontiers in cellular and infection microbiology - 1 Jan 2026

Shi Yuwen, He Jiakang, Shi Kaichuang, Yin Yanwen, Long Feng, Feng Shuping, Wei Zuzhang

Abstract excerpt

Background: Porcine reproductive and respiratory syndrome (PRRS) is a widely prevalent disease of reproductive failure of pregnant pigs and respiratory syndromes in pigs of different ages, especially in piglets. The etiological agents include PRRS virus (PRRSV) genotypes 1 (PRRSV-1) and PRRSV-2, whereas their clinical symptoms are similar and hard to differentiate. It is necessary to establish accurate and...

Topics

Join the communities discussing this publication.

DNA Primers
Genotype
Polymerase Chain Reaction
Porcine Reproductive and Respiratory Syndrome
Porcine respiratory and reproductive syndrome virus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Reproducibility of Results

Sensitivity and Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 41835007
DOI: 10.3389/fcimb.2026.1701517
PMCID: PMC12982388

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Development of a novel duplex crystal digital PCR for the detection of PRRSV-1 and PRRSV-2.PMID 41835007Read ↗

The updated duplex fluorescence quantitative RT-PCR assay for simultaneous detection of PRRSV-1 and PRRSV-2Semantic proximity Read ↗

Development and Implementation of a Quadruple RT-qPCR Method for the Identification of Porcine Reproductive and Respiratory Syndrome Virus StrainsSemantic proximity Read ↗

Development of a Multiplex RT-qPCR Method for the Identification and Lineage Typing of Porcine Reproductive and Respiratory Syndrome Virus.Semantic proximity Read ↗

Establishment and Application of a Quadruplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assay for Differentiation of Porcine Reproductive and Respiratory Syndrome Virus, Porcine Circovirus Type 2, Porcine Circovirus Type 3, and Streptococcus suisSemantic proximity Read ↗

A multiplex crystal digital PCR for detection and quantitation of porcine circovirus type 2 and type 3Semantic proximity Read ↗

Research Progress on the detection methods of porcine reproductive and respiratory syndrome virusSemantic proximity Read ↗

Development of a one-step reverse transcription-quantitative polymerase chain reaction assay for the detection of porcine reproductive and respiratory syndrome virusSemantic proximity Read ↗

A triplex crystal digital PCR for the detection of genotypes I and II African swine fever virusSemantic proximity Read ↗

Development of universal and quadruplex real‐time RT‐PCR assays for simultaneous detection and differentiation of porcine reproductive and respiratory syndrome virusesSemantic proximity Read ↗

Development of a Triplex Real-Time PCR Method for the Simultaneous Detection of Porcine Circovirus 2, 3, and 4 in China Between 2023 and 2024Semantic proximity Read ↗

Development of a TaqMan-Probe-Based Multiplex Real-Time PCR for the Simultaneous Detection of Porcine Circovirus 2, 3, and 4 in East China from 2020 to 2022Semantic proximity Read ↗

Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.